Biology 111 Principles of Biology Lab

 

Instructor: Dr. Carl S. Luciano

SECTION A04  MONDAY 1:25-4:15

 SECTION B04 WEDNESDAY, 4:40-7:15

FALL, 2008

 

I. Contact Information

 

Dr. Carl S. Luciano, 114 Weyandt,  357-2352, email: luciano@iup.edu

 

My office is behind the main office for the Biology Department, on the first floor of Weyandt near the Elkin Hall entrance to the building (where all the construction trucks pull in). Come in to the main office and ask the secretaries to come back in to my office area. You are encouraged to stop in at any time if you have a question or a problem with Principles of  Biology material.

 

If you have a question which can be answered over the phone, don’t hesitate to call the office. If you can’t reach me directly by phone, contact the Biology Department Office at 357-2352 and leave a message for me.

 

Use email to contact me when possible. I do read my email and reply.

 

Regular office hours are: 2:00-4:00 PM W and 1:30-2:30 F or by appointment-please email.

 

You may also contact me through the Biology Department Secretary Ms. Bertha Cecconi at 357-2352

 

 

II. Announcements, Notes, Messages

 

HERE ARE ELECTRONIC VERSIONS OF YOUR FINAL ASSIGNMENT SHEETS

 

Lab Report #3 (individual assignment)

Poster Presentation (group assignment)

 

 

       UPDATE on AUGUST 25, 2008

 

        MONDAY LAB SECTION MEETS TODAY!!!!!!!

 

       

III. Weekly Activities

        Week One (beginning August 25)

        Week Two (beginning September 8)

         Week Three (beginning September 15)

         Week Four (beginning September 22)

         Week Six (beginning October 6)

         Weeks Seven and Eight (beginning October 13)

       

IV. Lab Syllabus

 

        Go to the file containing the Lab Syllabus.

 

V. Handouts, Assignment Sheets and Rubrics

 

        Handouts:

                      The Scientific Method

                      Scientific Figures

                      Overview of Cells  (contains links)       

                      Writing up Your Results    (Sept. 8, 10)

                      Paper on Blood Plasma Proteins (Sept. 8, 10)

                      Paper on Wasp Venom Proteins (Sept. 8, 10)

                      Writing up Your Conclusions    (Sept. 15, 17)

                      Partial Purification of Acid Phosphatase    (Sept. 22, 24)

                      Writing up Materials and Methods    (Sept. 22, 24)

                      Lab Report #1    (October 6, 8)

                      Procedure for Daphnia    (October 13, 15, 20, 22)

 

 

 

 

VI. Links

 

Here are some useful links to background information of the Bradford (Bio-Rad) colorimetric protein assay

    

http://www-class.unl.edu/biochem/protein_assay/    Provides excellent introduction to Bradford and other assays
 
http://matcmadison.edu/biotech/resources/methods/labManual/unit_4/exercise_13.htm   Also some good background and a definition of colorimetric assays-contains

            a detailed protocol and a model table for constructing the experiment

 
http://www.ruf.rice.edu/~bioslabs/methods/protein/bradford.html  Brief background and description
 
http://www.piercenet.com/Products/Browse.cfm?fldID=02020105  Link to a commercial product

 

Here are some useful links to background information of the acid phosphatase enzyme assay

 

The first two links illustrate some research involving acid phosphatase

 

http://breast-cancer-research.com/content/3/2/E002

 

http://soil.scijournals.org/cgi/content/abstract/52/6/1612

 

The link below gives some background on the enzyme and the assay

 

http://www.bio.mtu.edu/campbell/bl482/lectures/lec2/482ex2a.htm

 

Here are some useful links to background information on the water flea Daphnia

 

 

Draft version of  instructions for Daphnia culturing along with a little bit of background

http://web.cecs.pdx.edu/~davidr/discus/articles/daphnia.html

 

Nice pictures as well as some links, discussion of use of Daphnia in ecological assays
http://ei.cornell.edu/toxicology/bioassays/daphnia/photos.asp

 

Microscopy site with links to videos-watch these before you do the experiment! 
http://www.microscopyu.com/moviegallery/pondscum/crustacean/daphnia/index.html

 

Teachers' site with short but high magnification video of Daphnia heart beating 
http://www.teachertube.com/view_video.php?viewkey=f9223de3684d74b53be0&page=1&viewtype=&category=

 

 

VII. Others

 

Please email your comments about my site directly to me.   luciano@iup.edu

Go backl to Luciano Home Page